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Where Do Organisms in the Hay Infusion Come From?

FAQs Jackson Bowman September 13, 2022

The microbes in the hay infusion may come from the dead grass that was put in the cup. Some of them came through the air that was channeled to the surface of the water table.

What organisms are found in hay infusion?

In the hay or on other dry plants, leaf litter etc. there are numerous resting stages of bacteria (e.g. spores of the so-called “hay bacterium” Bacillus subtilis), algae, other protozoa or rotifers and tardigrades . After a few days in the water, these organisms come back to life.

What happened to the microbes after a week in hay infusion?

After about a week, bacteria and protozoa should be visible under a light microscope. Most organisms can be observed at 200x magnification. The sugars in hay form the basis for a food web. Bacteria feed on the sugars and protozoa feed on the bacteria and each other.

Is diversity of microorganisms seen in the hay infusion?

The hay infusion contained an abundance of different species of protists, including flagellates, amoebas, and fungi (Figure 3 A, Figure S1). Most of the diversity was found in the family Ciliophora, so we designed and optimized a ciliate-specific ssu rRNA probe.

How do you make a hay infusion culture?

Take a handful of dried grass or hay (free from pesticides or herbicides) and cut the grass into smaller pieces. Put the cut grass in the cup and about 0.5-1 liter of water. Add 1-2 drops of milk. The water becomes slightly cloudy.

How do you collect protists?

A good way to catch them is to turn a container upside down until it is positioned just above the surface of the mud. Then slowly let the air out so that the top one layer is sucked into the glass. You can move the glass slowly as you tilt it, allowing you to collect from a larger area.

How do protozoa grow?

Boil a liter of pond, spring or rain water. When the water is boiling, add a small handful of hay (ideally timothy grass hay) and boil for another ten minutes . Boiling breaks down the hay and creates an ideal medium for bacteria to grow. Leave this mixture for two to three days.

Why is yeast added to hay infusion?

Next, why did we add the yeast? Yeasts are tiny fungi that multiply rapidly, feeding on the nutrients from dead grass. Yeast is excellent food for protozoa. Initially, the population will be unbalanced.

How long does a hay infusion take?

During incubation, check the infusion and add more pond water as it evaporates. In 5 to 10 days, the broth should turn dark and cloudy. Examination under a microscope reveals a large number of microorganisms.

What is hay infusion?

Hay infusions are nutrient media for growing ciliates, such as Paramecium, which are interesting microscopy specimens. A little dry grass (e.g. hay) is boiled with a little water. The water turns brownish-green and looks a bit like tea.

How do you collect microorganisms from pond water?

How do you get microscopic pond life?

The easiest way to collect small pond life and organisms is to squeeze water from plants or pond sludge into a container. Scrapping the growth of aquatic plants or anything covered in green or brown vegetation usually results in high levels of microscopic pond life.

References:

  1. https://www.bresser.de/c/en/support/guidebooks/mikroskopie/hay-infusion/
  2. https://www.carolina.com/teacher-resources/Interactive/make-a-hay-infusion/tr41608.tr
  3. https://www.researchgate.net/figure/Amoebae-found-in-the-hay-infusion-enrichment-are-closely-related-to-Naegleria-spp-The_fig3_51884732
  4. https://www.microbehunter.com/making-a-hay-infusion/
  5. http://www.microscopy-uk.org.uk/pond/collect.html
  6. https://microscope-microscope.org/microscope-applications/culturing-protozoa/
  7. https://thehappyscientist.com/content/microscopes-making-hay-infusion
  8. https://instr.bact.wisc.edu/book/displayarticle/2
  9. https://www.microbehunter.com/how-can-i-make-a-hay-infusion/
  10. https://www.youtube.com/watch?v=459E5Vm3P9Y
  11. http://blog.microscopeworld.com/2013/02/collecting-microscopic-pond-life.html

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